Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Medchemexpress LLC Tofacitinib 10 mM * 1 mL in DMSO, Solid + Solvent, Ready for Reconstitution
Tofacitinib is an orally available JAK3/2/1 inhibitor with IC50s of 1, 20, and 112 nM, respectively.
Revvity Health Sciences Inc HTRF Human Phospho-SLP76 (Tyr145) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-SLP76 (Tyr145) Detection Kit, 10,000 Assay Points
Alkali Scientific ASI™ 6-Well Confocal Plate (35m), Clear, Adhesion Type, Sterile, 4/Cs, PS/Flux, Treated (Positive-Charged/ Surface Coated), 50/Cs, 22 x 22 x 0.13mm, Hole 3.14mm
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products: Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy ▪ Bottom materials: Glass/FLux (no surface treatment for glass bottom)▪ Dish color: Clear▪ Dish size: 35 Ø▪ Plate size: 6well Plate▪ Hole size: 13 Ø / 20 Ø / 25Ø▪ Non – pyrogenic▪ Non – cytotoxic▪ DNase / RNase – free▪ Human DNA – free
Revvity Health Sciences Inc HTRF Human Total JAK2 Detection Kit, 50,000 Assay Points
HTRF Human Total JAK2 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 50,000 Assay Points
Alkali Scientific ASI™ 6-Well Confocal Plate (35m), Clear, Adhesion Type, Sterile, 4/Cs, PS/Glass, Non-Treated (Non-Charged/ Hydrophobic), 100/Cs, 22 x 22 x 0.13mm, Hole 3.14mm
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products: Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy ▪ Bottom materials: Glass/FLux (no surface treatment for glass bottom)▪ Dish color: Clear▪ Dish size: 35 Ø▪ Plate size: 6well Plate▪ Hole size: 13 Ø / 20 Ø / 25Ø▪ Non – pyrogenic▪ Non – cytotoxic▪ DNase / RNase – free▪ Human DNA – free
Molecular Devices Microplate Reader, Multi-Mode, Top/Bottom Read, 200-1000 nm
The SpectraMax M4 is a multi-mode microplate reader designed for absorbance, fluorescence, luminescence, and time-resolved fluorescence assays. It supports top and bottom reads and is engineered for flexible, routine laboratory measurements across a range of assay types.
- Multi-mode detection: absorbance, fluorescence, luminescence, TRF.
- Top and bottom reading for flexible assay configurations.
- Absorbance range 200-1000 nm, fluorescence/luminescence/TRF 250-850 nm.
- Dual-monochromator based detection for spectral flexibility.
- Compatible with standard microplate formats, including 96-well plates.
- Suitable for enzyme assays, nucleic acid and protein quantitation, and reporter assays.
Revvity Health Sciences Inc HTRF Human Total BRD2 Detection Kit, 10,000 Assay Points
HTRF Human Total BRD2 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human and Mouse Total RNA Pol II Detection Kit, 10,000 Assay Points
HTRF Human and Mouse Total RNA Pol II Detection Kit, 10,000 Assay Points