Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Fluorescence Polarization Filter, 635 nm, 35 nm, Vertical
Molecular Devices fluorescence polarization filter with holder designed for fluorescence polarization assays and FP-compatible detection on compatible multi-mode microplate readers. The filter is specified for a 635 nm center wavelength with a 35 nm bandwidth and is polarized to enable fluorescence polarization measurements.
- Designed for fluorescence polarization assays and FP detection modes.
- Center wavelength 635 nm with 35 nm bandwidth.
- Polarized to enable measurement of fluorescence anisotropy.
- Includes holder for installation into compatible filter assemblies.
- Intended as an accessory for multi-mode microplate readers that accept replaceable filters.
Molecular Devices Absorbance Filter, 350 nm, 10 nm Bandwidth
Molecular Devices 350 nm absorbance filter with 10 nm bandwidth provides narrowband absorbance detection for microplate assays. It is sold as a replaceable filter accessory for compatible absorbance microplate readers and is intended to improve selectivity for wavelength-specific absorbance measurements.
- Center wavelength: 350 nm
- Bandwidth (full width at half maximum): 10 nm
- Filter type: Absorbance
- Intended application: Narrowband absorbance measurements in microplate assays
- Compatibility: Designed for use in compatible absorbance microplate reader filter assemblies
Molecular Devices Absorbance Filter, 575 nm, 8 nm Bandwidth
This absorbance filter provides narrowband detection at a 575 nm center wavelength with an 8 nm full-width bandwidth for visible-range absorbance assays. It is designed for use in FilterMax F3 and F5 multi-mode microplate readers.
- Provides narrowband absorbance detection at 575 nm.
- 8 nm full-width bandwidth for selective wavelength measurement.
- Designed for use with FilterMax F3 and F5 multi-mode microplate readers.
- Suitable for visible-range absorbance assays and endpoint measurements.
Molecular Devices Fluorescence Polarization Filter, 465 nm, 35 nm, Polarized
Fluorescence polarization filter set designed for microplate fluorescence assays. Centered at 465 nm with a 35 nm bandwidth, the set provides vertically and horizontally polarized filters and includes a holder for installation in compatible microplate readers.
- Designed for fluorescence polarization measurements on microplate readers.
- Center wavelength 465 nm with a 35 nm bandwidth.
- Includes vertically and horizontally polarized filters for polarization assays.
- Supplied as a set of two filters with a single holder for installation.
- Enables selective excitation and improved signal specificity in fluorescence assays.
Electron Microscopy Sciences MultiLap™ Fish Otolith Polishing Tool 6-Position Alignment Fixture
For use with Model 195 - 6 Position MultiLap (50168-95).
Molecular Devices Excitation Filter, 585 nm, 10 nm Bandwidth
This excitation filter provides narrowband fluorescence excitation centered at 585 nm with a 10 nm full width, for use in compatible multi-mode microplate readers.
- Center wavelength 585 nm.
- Bandwidth 10 nm full width.
- Excitation filter type suitable for narrowband fluorescence assays.
- Designed for installation in standard filter slide assemblies.
Revvity Health Sciences Inc HTRF XIAP BIR3 Binding Kit Red Ligand, 1 Vial
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Revvity Health Sciences Inc HTRF Human Total ATG14 Detection Kit, 10,000 Assay Points
HTRF Human Total ATG14 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total FGFR3 Detection Kit, 50,000 Assay Points
HTRF Human Total FGFR3 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD4 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total BRD4 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total H2AX Detection Kit, 10,000 Assay Points
HTRF Human Total H2AX Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CHK-1 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total CHK-1 Detection Kit, 500 Assay Points